Alpha-tubulin Antibody | 7599

Alpha-tubulin Antibody | 7599 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Pig: (100%) , Chicken: (100%)

Immunogen: Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin.
The immunogen is located within the first 50 amino acids of Alpha-tubulin.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Alpha-tubulin antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 50 kDa
Observed: 45 kDa

Validation: N/A

Isoform: N/A

Purification: Alpha-tubulin antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Alpha-tubulin antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Alpha-tubulin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Alpha-tubulin Antibody: TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1) . Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2) . Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3) . Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.