AMPKα2 (Phospho-S345) polyclonal Antibody | BS94020

AMPKα2 (Phospho-S345) polyclonal Antibody | BS94020 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Application: WB ICC/IF IHC

Application Range: WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100

Background: Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic α subunit, and regulatory β and γ. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 gene maps to chromosome 5p12 and encodes a 548 amino acid protein. The major regulatory site phosphorylated by AMPKK on AMPKα has been identified as Thr 172 within the activation loop between the DFG and APE motifs of the alpha-subunits.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: AMPKα2 (Phospho-S345) polyclonal Antibody detects endogenous levels of AMPKα2 protein only when phosphorylated at S345.

Molecular Weight: 62 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: AMPK subunit alpha-2, ACACA kinase, HMGCR kinase, PRKAA2, 5'-AMP-activated protein kinase catalytic subunit alpha-2, AAPK2, ACACA kinase, Acetyl-CoA carboxylase kinase, AMPK alpha 2 chain, AMPK subunit alpha-2, AMPK2, AMPKa2, AMPKalpha2, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA, PRKAA2, Protein kinase AMP activated alpha 2 catalytic subunit, Protein kinase AMP activated catalytic subunit alpha 2,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human AMPK alpha 2.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: ProA affinity purified

Pathway: