ANAPC5 Antibody | 22-706

ANAPC5 Antibody | 22-706 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2) .

Research Area: Cell Cycle

Tested Application: WB, IHC, IP

Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:50 - 1:100

Specificiy: N/A

Positive Control 1: SW620

Positive Control 2: MCF-7

Positive Control 3: HL-60

Positive Control 4: 22Rv1

Positive Control 5: A-549

Positive Control 6: Mouse spleen

Molecular Weight: Observed: 85kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: APC5 Antibody: APC5, APC5, Anaphase-promoting complex subunit 5, Cyclosome subunit 5

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C) , a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b) , compared to isoform a.