Annexin A1 Antibody (Center) [APR14932G]

Annexin A1 Antibody (Center) [APR14932G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This Annexin A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the Central region of human Annexin A1.

Cellular Localisation: Nucleus. Cytoplasm. Cell projection, cilium {ECO:0000250|UniProtKB:P46193}. Cell membrane. Membrane; Peripheral membrane protein. Endosome membrane {ECO:0000250|UniProtKB:P07150}; Peripheral membrane protein {ECO:0000250|UniProtKB:P07150}. Basolateral cell membrane {ECO:0000250|UniProtKB:P51662}. Apical cell membrane {ECO:0000250|UniProtKB:P10107}. Lateral cell membrane {ECO:0000250|UniProtKB:P10107}. Secreted. Secreted, extracellular space. Cell membrane; Peripheral membrane protein; Extracellular side. Secreted, extracellular exosome. Cytoplasmic vesicle, secretory vesicle lumen. Cell projection, phagocytic cup {ECO:0000250|UniProtKB:P10107}. Early endosome {ECO:0000250|UniProtKB:P19619}. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:P19619}; Peripheral membrane protein {ECO:0000250|UniProtKB:P19619}. Note=Secreted, at least in part via exosomes and other secretory vesicles. Detected in exosomes and other extracellular vesicles (PubMed:25664854) . Alternatively, the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in the protein translocation from the cytoplasm into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059) . Detected in gelatinase granules in resting neutrophils (PubMed:10772777) . Secretion is increased in response to wounding and inflammation (PubMed:25664854) . Secretion is increased upon T-cell activation (PubMed:17008549) . Neutrophil adhesion to endothelial cells stimulates secretion via gelatinase granules, but foreign particle phagocytosis has no effect (PubMed:10772777) . Colocalizes with actin fibers at phagocytic cups (By similarity) . Displays calcium-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678) {ECO:0000250|UniProtKB:P10107, ECO:0000269|PubMed:10772777, ECO:0000269|PubMed:17008549, ECO:0000269|PubMed:2532504, ECO:0000269|PubMed:25664854, ECO:0000269|PubMed:32272059, ECO:0000269|PubMed:8557678}

Molecular Weight: 38714

Clone: Polyclonal

Gene Name: ANXA1

Gene ID: 301

Function: Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544) . Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity) . Promotes resolution of inflammation and wound healing (PubMed:25664854) . Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:25664854) . Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149) . Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T- cells (PubMed:17008549) . Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549) . Has no effect on unstimulated T cells (PubMed:17008549) . Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149) . Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660) . Has high affinity for Ca (2+) and can bind up to eight Ca (2+) ions (By similarity) . Displays Ca (2+) -dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678) . Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca (2+) -dependent interaction between phagosomes and the actin cytoskeleton (By similarity) .

Summary: Tissue Location: Detected in resting neutrophils (PubMed:10772777) . Detected in peripheral blood T-cells (PubMed:17008549) . Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors (PubMed:25664854) Detected in placenta (at protein level) (PubMed:2532504) . Detected in liver.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, FC

Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50

Synonyms: Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35, ANXA1, ANX1, LPC1