Anti-CoV-2 & SARS-CoV S1 Antibody (Clone# CR3022) | A2103

Coronaviruses (CoV) are a large group of enveloped positive-sense RNA viruses. They belong to subfamily Coronavirinae, in the family of Coronaviridae, of the order of Nidovirales. The Coronavirus genome is about 30 kb in length and encodes four structural proteins, namely, spike (S), envelope (E), membrane (M) and nucleocapsid (N), multiple nonstructural proteins and other accessory proteins. The Spike (S) protein is a 180 kDa type I glycosylated transmembrane protein, that assembles as a trimer on the surface of the virus, hence the coronavirus appears crown-shaped (In Latin, corona means crown). The ectodomain of the Spike protein consists of two domains: S1 domain whose main function is receptor binding, and S2 domain whose main function is membrane fusion. S1 binds to the cell surface receptor of host for attachment during the viral entry, this induces conformational change in the S2, thus enabling the fusion of host and viral membranes and ultimately entry of the viral genome in the host cell.

A2103 | Anti-CoV-2 & SARS-CoV S1 Antibody (Clone# CR3022) DataSheet

Antibody Target: CoV-2 & SARS-CoV Spike S1

Target Alternative Name: Spike protein; COVID19; COVID 19; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV

Tag Line: Recombinant monoclonal antibody to detect the expression of SARS-CoV-2 and SARS-CoV Spike S1 protein. This antibody was manufactured using recombinant platform with variable regions from B-cell clone CR3022. The monoclonal antibody, originally generated by sequencing peripheral blood lymphocytes of SARS-CoV infected patient, can bind to SARS-CoV-2 RBD at a KD of 6.3 nM.

Category: Primary antibody

Host: Recombinant

Isotype: Human IgG1, kappa

Species Reactivities: Rat,Mouse,Human

Immunogen Sequence: This antibody binds the amino acids 300-500 in the S1 domain of the SARS-CoV as well as SARS-CoV-2 Spike protein. The binding epitope is only accessible in the "open" conformation of the spike protein

Accession #: P59594

Gene ID:

Appearance: Colorless liquid

Form: Liquid

Concentration:

Formulation: In PBS with 0.02% Proclin 300

Purification: Protein A affinity purified

Application: Immunohistochemistry (IHC),Western Blotting

Positive Control:

Application And Usages: WB 1:500 - 1:2000, IHC 1:50 - 1:200

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: FALSE

Immunocytochemistry Verified: FALSE

Immunofluorescence Verified: FALSE

Immunoprecipitation Verified: FALSE

FACS Verified: FALSE

ELISA Verified: FALSE

ChIP Verified: FALSE

Dot Blot Verified: FALSE

Flow Cytometry Verified: FALSE