Anti- CUL7 Antibody | FNab02081

Anti- CUL7 Antibody | FNab02081 | Gentaur UK, US & Europe Distribution

Form: liquid

Purification: Immunogen affinity purified

Purity: ≥95% as determined by SDS-PAGE

Host: Rabbit

Clonality: polyclonal

Clone ID: N/A

Isotype: IgG

Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Background: Core component of the 3M and Cul7-RING (FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed:24793695) . Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5 (PubMed:24793696) . Core component of a Cul7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1 (PubMed:21572988, PubMed:24362026) . Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain (PubMed:21572988) . Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING (FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2) (PubMed:18498745) . The Cul7-RING (FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradatation, thereby affecting cell proliferation and differentiation (PubMed:24362026) . Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development (PubMed:20139075) . Does not promote polyubiquitination and proteasomal degradation of p53/TP53 (PubMed:16547496, PubMed:17332328) . While the Cul7-RING (FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING (FBXW8) complex may be have additional functions.

Immunogen: cullin 7

synonyms: KIAA0076

Molecular weight (observed) : 185kd

Reactivity Species: Human, Mouse

Tested Application: ELISA, WB, IF, IP

Recomended Dillution : WB: 1:500-1:2000; IHC: 1:20-1:200

Research Area: Metabolism