Anti-H3K36me3 Antibody | A2031

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack poly A tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

A2031 | Anti-H3K36me3 Antibody DataSheet

Antibody Target: H3K36me3

Target Alternative Name: H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A

Tag Line: Affinity purified rabbit polyclonal antibody to detect trimethylation of Histone H3 at Lysine 36 in human, mouse and rat samples

Category: Antibody

Host: Rabbit

Isotype: IgG

Species Reactivities: Rat, Mouse, Human

Immunogen Sequence: A synthetic methylated peptide targeting residues around Lysine 36 of human Histone H3

Accession #: Q16695

Gene ID: 8290

Appearance: Colorless liquid

Form: Liquid

Concentration:

Formulation: In PBS with 0.02% sodium azide, 50% glycerol, pH 7.3

Purification: Affinity purified

Application: ChiP, Immunohistochemistry (IHC), Immunofluorescence (IF), Dot Blot, Western Blotting

Positive Control:

Application And Usages: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, ChIP 1:20 - 1:100

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Western blot data verified using HeLa, C2C12 cells and H3 protein extracts

Immunocytochemistry Verified: True

Immunofluorescence Verified: True

Immunoprecipitation Verified: False

FACS Verified: False

ELISA Verified: False

ChIP Verified: True

Dot Blot Verified: True

Flow Cytometry Verified: False