ELISA: The anti HA diluted 1:70.000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.
Synthetic peptide corresponding to Influenza A HA tag conjugated to keyhole limpet haemocyanin. Influenza hemagglutinin-HA-epitope
Positive control
WB: 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein). IP: Nuclear lysate of HEK-293T cells transiently expressing HA-tagged protein. ICC/IF: U-2 cells. Mouse olineu cells. ChIP: Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS.
General notes
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