Anti-HDAC6 Monoclonal Antibody (D1 domain-specific) | A1486

The affinity purified mouse monoclonal antibody is immunospecific for the D1 domain (a.a. 71-91) of human histone deacetylase 6 (HDAC6) as determined by ELISA and peptide library screening. HDAC6 belongs to the class II of the histone deacetylase family (EC 3.5.1.98). The protein of molecular mass 131 kDa consists of two catalytic domains, the serine-glutamate-rich SE14 domain and the ubiquitin-binding ZnF domain. HDAC6 shuttles between the nucleus and the cytoplasm, but its localization is predominantly cytoplasmic. The enzyme deacetylates various substrates including ?-tubulin, HSP90, cortactin, histones and other proteins. Described protein plays an important role in microtubule-dependent cell motility, apoptosis, cell signaling and transcriptional regulation, protein degradation, immune response and DNA repair. HDAC6 is linked to several neuronal diseases and carcinogenesis

A1486 | Anti-HDAC6 Monoclonal Antibody (D1 domain-specific) DataSheet

Antibody Target: HDAC6

Target Alternative Name: KIAA0901, JM21, HD6, HDAC6-D1

Tag Line: Mouse monoclonal antibody to detect HDAC6 (D1 Domain) in human samples

Category: Epigenetics

Host: Mouse

Isotype: IgG

Species Reactivities: Human

Immunogen Sequence: Human Histone deacetylase 6 (HDAC6; sequence NP_006035.2, UniProtKB - Q9UBN7)

Accession #: Q9UBN7

Gene ID: 10013

Appearance: Colorless Liquid

Form: Liquid

Concentration:

Formulation: 1 mg/ml in PBS, pH 7.4, 0.02% sodium azide

Purification: Affinity purified from hybridoma culture supernatant (Protein A)

Application: ELISA, IC/IF, WB, IP

Positive Control: HEK293T cells

Application And Usages: ELISA: 0.5 – 5µg/ml; Immunocytochemistry/Immunofluorescence: 5 – 50 µg/ml; WB: 0.5-5µg/ml; IP: 1 – 2 µg mAb/1 µg purified HDAC6

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans

Handling: The antibody solution should be gently mixed before use

Western Blot Verified: False.5 – 5µg/ml

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: 5 – 5False µg/ml

Immunoprecipitation Verified: 1 – 2 µg mAb/1 µg purified HDAC6

FACS Verified: Not Verified

ELISA Verified: False.5 – 5µg/ml

ChIP Verified: Not Verified

Dot Blot Verified: Not Verified

Flow Cytometry Verified: Not Verified