This antibody recognizes only the non-phosphorylated C-terminus of CXCR4 (residues 341-352). Phosphorylation of S346/347 blocks antibody binding. PMID: 24154522, 25451233.

We recommend dephosphorylation of samples using lambda phosphatase treatment. Please refer to application notes.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• WB: Jurkat whole cell lysate. IF/ICC: Jurkat and Ramos cells. IHC-P: Retina and brain of E14 mouse embryo, Human small cell lung carcinoma tissue. Flow Cyt (intra): Jurkat cells.

This product is a recombinant monoclonal antibody, which offers several advantages including:

• - High batch-to-batch consistency and reproducibility
• - Improved sensitivity and specificity
• - Long-term security of supply
• - Animal-free production