Anti-IDH1 (Isocitrate Dehydrogenase 1) monoclonal Antibody | MB62276

Anti-IDH1 (Isocitrate Dehydrogenase 1) monoclonal Antibody | MB62276 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat

Application: IF, WB

Application Range: WB 1:2000, IHC 1:50, IF 1:100

Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+) -dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+) -dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Storage & Stability: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Specificity: isocitrate dehydrogenase (NADP (+) ) 1, cytosolic

Molecular Weight: 46.7 kDa (Predicted)

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD

Immunogen: Full-length protein expressed in 293T cell transfected with human IDH1 expression vector

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Pathway: N/A