Anti-LIMK1 Antibody | A2228

LIMK1 is a serine-threonine protein kinase that contains 2 zinc finger motifs, known as LIM motifs at their N-terminus and a kinase sequence at their C terminus. The protein is expressed ubiquitously in different regions of the adult brain. It has reduced expression in the heart and skeletal muscles. LIMK1 prevents the cleavage of actin filament and stabilizes the actin cytoskeleton by phosphorylating and inactivating actin binding factors such as cofilin-1 (CFL1), cofilin-2 (CFL2), and destrin (DSTN). Additionally, it acts downstream of Rho family GTPase signal transduction pathways. Kinases such as ROCK1, PAK1, and PAK4 phosphorylate LIMK1 on the threonine residue in the activation loop and increase the activity of LIMK1. LIMK1 deletion is associated with the impairment of visuospatial cognition and long-term memory of Williams syndrome. Overexpression of LIMK1 is observed in multiple cancers such as breast, colorectal, and prostate cancer.

A2228 | Anti-LIMK1 Antibody DataSheet

Antibody Target: LIMK1

Target Alternative Name: LIMK, LIM domain kinase 1, LIMK-1, EC 2.7.11.1, LIM Motif-Containing Protein Kinase, LIMK1

Tag Line: Affinity purified rabbit polyclonal antibody to detect LIMK1 expression in human, mouse and rat samples

Category: Primary antibody

Host: Rabbit

Isotype: IgG

Species Reactivities: Rat,Mouse,Human

Immunogen Sequence: KLH-conjugated synthetic peptide targeting a sequence within the center region of human LIMK1

Accession #: P53667

Gene ID: 3984

Appearance: Colorless liquid

Form: Liquid

Concentration:

Formulation: In 0.42% Potassium phosphate; 0.87% NaCl; pH 7.3; 30% glycerol; and 0.01% sodium azide

Purification: Affinity purified

Application: Immunofluorescence (IF),Western Blotting

Positive Control:

Application And Usages: WB 1:500 - 1:1000, IF 1:100 - 1:500

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: TRUE

Immunocytochemistry Verified: FALSE

Immunofluorescence Verified: TRUE

Immunoprecipitation Verified: FALSE

FACS Verified: FALSE

ELISA Verified: FALSE

ChIP Verified: FALSE

Dot Blot Verified: FALSE

Flow Cytometry Verified: FALSE