Anti-mCherry Polyclonal Antibody | AB0081

Goat Anti-mCherry IgG Polyclonal Antibody | AB0081 | Antibodies

Overview: Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Other Names: Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Other Names: Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Concentration: 3 mg/ml

Antibody Type: Primary

Target Type: anti-mCherry

Accession Number: N/A

Host Species: Goat

Immunogen Species: N/A

Immunogen Type: Recombinant protein

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Clonality: Polyclonal

Confirmed Reactive Species: mCherry, tdTomato, RFP

Specificity Detail: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody (AB0081) recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Buffer: PBS, 20% glycerol and 0.05% sodium azide

Purification: Epitope affinity purified

Conjugation: Unconjugated

Format: N/A

Application: WB, IF, IHC-P, IHC-F, IEM

Concentration/Dilution: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Storage: N/A

References: 1. Luo H, Liu HZ, Zhang WW, et al. Cell Rep. 2019 Nov. PMID:31747607, 2. Stewart S, Le Bleu HK, Yette GA, et al. bioRxiv Oct 2019, 3. Marin-Mogollon C, Salman AM, Koolen KMJ, et al. Front Cell Infect Microbiol. 2019 Apr. PMID:31058097, 4. Saito YC, Maejima T, Nishitani M, et al. J Neurosci. 2018 Jul. PMID:29915137, 5. Cattaud V, PhD Thesis, University of Tolouse, France 2018, 6. Raven AP, PhD Thesis, Edinburg University, United Kingdom 2018, 7. Saito K, Nobuhisa I, Harada K et al. Exp Cell Res. 2018 Feb. PMID:29458175, 8. Soya S, Takahashi TM, McHugh TJ, et al. Nat Commun. 2017 Nov. PMID:29151577, 9. Fiuza M, Rostosky CM, Parkinson GT,et al. J Cell Biol. 2017 Aug. PMID:28855251, 10. Hasegawa E, Maejima T, Yoshida T et al. Proc Natl Acad Sci U S A. 2017 Apr. PMID:28396432, 11. Zhang-Hooks Y, Agarwal A, Mishina M, et al. Neuron. 2016 Jan. Supplemental Information, PMID:26774161, 12. Stykel MG, MSc Thesis, University of Calgary 2016, 13. Nakatsukasa K, Nishimura T, Byrne SD, et al. Mol Cell. 2015 Jul, Supplemental Information, PMID: 25982115, 14. Julkowska MM, McLoughlin F, Galvan-Ampudia CS, et al. Plant Cell Environ. 2015 Mar, PMID: 25074439, 15. Okorocha AE, PhD Thesis, University of Leicester, United Kingdom 2015, 16. Déglon N, Merienne N, Genome editing for the treatment of huntington's disease, EP 2982758 A1 Patent, 2014, 17. Benske A, MSc Thesis, The University of British Columbia, Canada 2014