Anti- MSH2 Antibody | FNab05371

Anti- MSH2 Antibody | FNab05371 | Gentaur UK, US & Europe Distribution

Form: liquid

Purification: Immunogen affinity purified

Purity: ≥95% as determined by SDS-PAGE

Host: Rabbit

Clonality: polyclonal

Clone ID: N/A

Isotype: IgG

Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Background: Component of the post-replicative DNA mismatch repair system (MMR) . Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Immunogen: mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

synonyms: COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2

Molecular weight (observed) : 105 kDa

Reactivity Species: Human, Mouse, Rat

Tested Application: ELISA, WB

Recomended Dillution : WB: 1:1000-1:4000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100

Research Area: Epigenetics, Metabolism, Cancer, Immunology, Developmental biology