710
Anti- PCNA Antibody | FNab06217
- SKU:
- 710-FNab06217-GEN
- Availability:
- In Stock - Delivery within a week
Description
Anti- PCNA Antibody | FNab06217 | Gentaur UK, US & Europe Distribution
Form: liquid
Purification: Protein A+G purification
Purity: ≥95% as determined by SDS-PAGE
Host: Mouse
Clonality: monoclonal
Clone ID: 8F9
Isotype: IgG1
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902) . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Immunogen: proliferating cell nuclear antigen
synonyms: Proliferating cell nuclear antigen, Cyclin
Molecular weight (observed) : 38 kDa
Reactivity Species: Human, Mouse, Rat, rabbit
Tested Application: ELISA, WB, IHC, IP
Recomended Dillution : WB: 1:5000-1:50000; IP: 1:200-1:1000; IHC: 1:500-1:2000; IF: 1:50-1:500
Research Area: Epigenetics, Metabolism, Immunology, Cancer, Cell Division and Proliferation