Anti-PD-1 Biotinylated Antibody | A1996

Programmed death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PD-1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-?. PD-L2 expression is more restricted and is expressed mainly by dendritic cells. Elevated levels of PD-1 inhibit the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-? by suppressing the activation and transduction of PI3K/AKT pathway. In addition, co-ligation of PD-1 inhibits BCR-mediating signal by de-phosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1 ligand results in reduced T cell proliferation and IFN-? secretion.

A1996 | Anti-PD-1 Biotinylated Antibody DataSheet

Antibody Target: Biotinylated PD-1

Target Alternative Name: CD279, PDCD1, PD1, hPD-1, hPD-l, SLEB2

Tag Line: Affinity purified rabbit polyclonal antibody to detect biotinylated PD-1 expression in human, mouse and rat samples

Category: Immune Checkpoint

Host: Rabbit

Isotype: IgG

Species Reactivities: Rat, Mouse, Human

Immunogen Sequence: Human PD-1 recombinant protein

Accession #: Q15116

Gene ID: 5133

Appearance: Colorless liquid

Form: Liquid

Concentration:

Formulation: In PBS with 30% glycerol, 0.5% BSA, 0.5mM EDTA, 0.03% Proclin, pH 7.2

Purification: Affinity purified

Application: Western Blotting, ELISA

Positive Control:

Application And Usages: WB 1:50-200, ELISA 1:500

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: True

Immunocytochemistry Verified: False

Immunofluorescence Verified: False

Immunoprecipitation Verified: False

FACS Verified: False

ELISA Verified: True

ChIP Verified: False

Dot Blot Verified: False

Flow Cytometry Verified: False