Anti- PUM2 Antibody | FNab06955

Anti- PUM2 Antibody | FNab06955 | Gentaur UK, US & Europe Distribution

Form: liquid

Purification: Immunogen affinity purified

Purity: ≥95% as determined by SDS-PAGE

Host: Rabbit

Clonality: polyclonal

Clone ID: N/A

Isotype: IgG

Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Background: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187) . Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276) . Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:22345517) . Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517) . Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845) . Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866) . May regulate DCUN1D3 mRNA levels (PubMed:25349211) . May support proliferation and self-renewal of stem cells.

Immunogen: pumilio homolog 2 (Drosophila)

synonyms: KIAA0235, PUMH2

Molecular weight (observed) : 140 kDa

Reactivity Species: Human, Mouse, Rat

Tested Application: ELISA, WB, IHC, IF, IP

Recomended Dillution : WB: 1:500-1:2000

Research Area: Stem Cells, Immunology, Metabolism