Anti-SARS-CoV-2 NSP5 Antibody | A2257

Severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) is an infectious disease caused by a novel beta-coronavirus called SARS-CoV-2. The SARS-CoV-2 is a large enveloped, positive-sense RNA with a genome about ~ 30 kb long. The genome encodes 14 open reading frames, of which ORF1a and ORF1b are translated to 2 polyproteins. The 2 polyproteins undergo autoproteolysis to yield 16 non-structural proteins (NSP1-16). The NSP5 protein, also known as 3C-like main protease (3CLpro) or main protease (Mpro) is a cysteine protease that plays an important role in viral protein synthesis. It possesses a cysteine-histidine catalytic dyad at its active site. The protease activity of NSP5 enables cleaving of the coronavirus polyprotein at 11 distinct sites. As a result of this cleaving activity, multiple mature and intermediate non-structural viral proteins are formed. NSP5 SARS-CoV-2 shares a sequence identity of 96.1% and sequence similarity of 99.7% with the SARS-CoV NSP5. NSP5 consists of 3 domains; domain I and II, both form ?-barrel chymotrypsin-like fold that is essential for its protease activity, and domain III, which is required for the oligomerization of the protein. Since NSP5 plays a critical role in the processing of coronavirus polyproteins, it is considered a potential drug target for the treatment of coronavirus infections.

A2257 | Anti-SARS-CoV-2 NSP5 Antibody DataSheet

Antibody Target: NSP5

Target Alternative Name: NSP5, 3C-like proteinase, 3CL-PRO,3CLp, Main protease, Mpro, Non-structural protein 5, SARS coronavirus main proteinase, Replicase polyprotein 1a

Tag Line: Rabbit polyclonal antibody to detect SARS-CoV-2 NSP5 protein

Category: Primary antibody

Host: Rabbit

Isotype: IgG

Species Reactivities: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Immunogen Sequence: Recombinant SARS-CoV-2 NSP5

Accession #: P0DTC1

Gene ID:

Appearance: Colorless liquid

Form: Liquid

Concentration:

Formulation: In PBS, pH 7.4, 50% glycerol, 0.05% proclin 300

Purification:

Application:

Positive Control:

Application And Usages:

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: FALSE

Immunocytochemistry Verified: FALSE

Immunofluorescence Verified: FALSE

Immunoprecipitation Verified: FALSE

FACS Verified: FALSE

ELISA Verified: FALSE

ChIP Verified: FALSE

Dot Blot Verified: FALSE

Flow Cytometry Verified: FALSE