Anti-SARS-CoV-2 Spike glycoprotein antibody (D1153) [APR33686G]

Anti-SARS-CoV-2 Spike glycoprotein antibody (D1153) [APR33686G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: N/A

Specificity: This Anti-SARS-CoV-2 Spike glycoprotein antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1149-1176 amino acids from the N-terminal region of human SARS-CoV-2 Spike glycoprotein.

Cellular Localisation: Virion membrane {ECO:0000255|HAMAP-Rule:MF_04099, ECO:0000269|PubMed:32979942}; Single-pass type I membrane protein {ECO:0000255|HAMAP-Rule:MF_04099}. Host endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000255|HAMAP-Rule:MF_04099}; Single-pass type I membrane protein {ECO:0000255|HAMAP-Rule:MF_04099} Host cell membrane {ECO:0000255|HAMAP-Rule:MF_04099}; Single-pass type I membrane protein {ECO:0000255|HAMAP-Rule:MF_04099}. Note=Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion (By similarity) . An average of 26 +/-15 S trimers are found randomly distributed at the surface of the virion (PubMed:32979942) . {ECO:0000255|HAMAP-Rule:MF_04099, ECO:0000269|PubMed:32979942}

Molecular Weight: 141178

Clone: Polyclonal

Gene Name: S {ECO:0000255|HAMAP-Rule:MF_04099}

Gene ID: 43740568

Function: [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32221306, PubMed:32075877, PubMed:32155444) . Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell (PubMed:33082294, PubMed:33082293) . This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2 (PubMed:33082293) . The stalk domain of S contains three hinges, giving the head unexpected orientational freedom (PubMed:32817270) . Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651) . Can be alternatively processed by host furin (PubMed:32362314) . Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: E

Dilution: N/A

Synonyms: N/A