Anti- SIRT4 Antibody | FNab07882

Anti- SIRT4 Antibody | FNab07882 | Gentaur UK, US & Europe Distribution

Form: liquid

Purification: Immunogen affinity purified

Purity: ≥95% as determined by SDS-PAGE

Host: Rabbit

Clonality: polyclonal

Clone ID: N/A

Isotype: IgG

Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Background: Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase (PubMed:19220062) . Catalyzes more efficiently removal of lipoyl-and biotinyl-than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879) . Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573) . In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation (PubMed:23663782) . Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782) . Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (PubMed:23746352) . Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD (+) levels (PubMed:24043310, PubMed:20685656) . Down-regulates insulin secretion (By similarity) .

Immunogen: sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)

synonyms: Sir2l4

Molecular weight (observed) : 35 kDa

Reactivity Species: Human, Mouse

Tested Application: ELISA, WB, IHC, IF

Recomended Dillution : WB: 1:500-1:2000; IHC: 1:20-1:200

Research Area: Epigenetics, Metabolism