Anti-TIGIT, Rabbit IgG, kappa Antibody | A2018

Recombinant monoclonal antibody to TIGIT. T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3, is a type I transmembrane protein, which belongs to the PVR family of immunoglobulin-like domain containing proteins. It is composed of an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on activated T cells, follicular T helper, memory T cells, and regulatory T cells and NK cells. TIGIT binds to CD155 (PVR) with high affinity and it regulates T-cell mediated immunity via the CD226/TIGIT-PVR pathway. TIGIT also serves as a negative regulator of NK and T cell activation.

A2018 | Anti-TIGIT, Rabbit IgG, kappa Antibody DataSheet

Antibody Target: TIGIT

Target Alternative Name: T Cell Immunoreceptor With Ig And ITIM Domains; V-Set And Immunoglobulin Domain Containing Protein 9; V-Set And Transmembrane Domain Containing Protein 3; VSIG9; VSTM3; T Cell Immunoreceptor With Ig And ITIM Domains; Washington University Cell Adhesion Molecule; WUCAM

Tag Line: Recombinant monoclonal antibody manufactured using Recombinant Platform with variable regions from the hybridoma 4D4 to detect TIGIT expression in mouse. 4D4 is an agonistic anti-TIGIT antibody, and addition of anti-CD3/anti-CD28 stimulated T cells to plate bound 4D4 is known to inhibit their proliferation. Further, 4D4 has been used in order to identify genes that are downstream of TIGIT signaling in Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue.

Category: Biosimilars

Host: Recombinant

Isotype: Rabbit IgG, kappa

Species Reactivities: Mouse

Immunogen Sequence: Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT medium and supernatants were screened for specificity by ELISA and FC using TIGIT-transfectants

Accession #: P86176

Gene ID: 201633

Appearance: Colorless liquid

Form: Liquid

Concentration: 1 mg/ml

Formulation: In PBS with 0.02% Proclin 300

Purification: Protein A affinity purified

Application: Agonist, Flow Cytometry

Positive Control:

Application And Usages: Agonist, FC

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: False

Immunocytochemistry Verified: False

Immunofluorescence Verified: False

Immunoprecipitation Verified: False

FACS Verified: False

ELISA Verified: True

ChIP Verified: False

Dot Blot Verified: False

Flow Cytometry Verified: False