AP2A1 Antibody | 43-204

AP2A1 Antibody | 43-204 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse

Homology: Expected Species Reactivity based on sequence homology: Rat, Pig, Cow

Immunogen: The immunogen for this antibody is: C-ELEPPAPESPMALLADPAPAAD

Research Area: Immunology, Stem Cell

Tested Application: E, WB, IHC, ICC

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kDa according to NP_055018.2) . The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 5ug/ml.Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.

Specificiy: This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Approx 110kDa

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: AP2A1, adaptor-related protein complex 2, alpha 1 subunit, ADTAA, AP2-ALPHA, CLAPA1, 100 kDa coated vesicle protein A, AP-2 complex subunit alpha-1, adapter-related protein complex 2 alpha-1 subunit, adaptin, alpha A, adaptor protein complex AP-2 subunit alpha-1, alpha-adaptin A, alpha1-adaptin, clathrin assembly protein complex 2 alpha-A large chain, clathrin-associated/assembly/adaptor protein, large, alpha 1, plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

User Note: N/A

BACKGROUND: N/A