AP3S1 Antibody | 6391

AP3S1 Antibody | 6391 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: AP3S1 antibody was raised against a 19 amino acid synthetic peptide near the center of human AP3S1.
The immunogen is located within the first 50 amino acids of AP3S1.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB, IHC-P, IF

Application: AP3S1 antibody can be used for detection of AP3S1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of AP3S1 are known to exist; this antibody will detect the two larger isoforms. AP3S1 antibody is predicted to not cross-react with other AP3 protein family members.

Positive Control 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: AP3S1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: AP3S1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: AP3S1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: AP3S1 Antibody: CLAPS3, Sigma3A, CLAPS3, AP-3 complex subunit sigma-1, AP-3 complex subunit sigma-3A, Sigma3A-adaptin

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: AP3S1 Antibody: AP3S1 belongs to the adaptor complexes 3 (AP3) small subunit family which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3 is an heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2) , a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2) . AP3S1 Interacts with AGAP1 and may play an important role in carcinoma.