Apaf1 Antibody | 2013

Apaf1 Antibody | 2013 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1.
The sequences of the immunogenic peptide are identical between human and mouse.
The immunogen is located within the first 50 amino acids of Apaf1.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, IHC-P, IF

Application: Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 μg/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1204 - K562 Cell Lysate

Positive Control 2: Cat. No. 10-501 - Human Heart Tissue Slide

Positive Control 3: Cat. No. 17-004 - K-562 Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 132 kDa
Observed: 130 kDa

Validation: N/A

Isoform: N/A

Purification: Apaf1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Apaf1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Apaf1 Antibody: CED4, APAF-1, KIAA0413, Apoptotic protease-activating factor 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Apaf1 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) . Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3) , which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.