APAF1 Antibody | XW-7034

APAF1 Antibody | XW-7034 | Gentaur UK, US & Europe Distribution

Host: Chicken

Reactivity: Human, Mouse

Homology: N/A

Immunogen: 307-388

Research Area: Apoptosis, Cancer

Tested Application: WB

Application: Apaf-1 antibody can be used for the detection of Apaf-1 by Western blot, may also work for IHC and ICC.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 141.8 kDa (calculated)

Validation: N/A

Isoform: N/A

Purification: Antigen affinity-purified

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Phosphate-Buffered Saline. No preservatives added.

Concentration: 1 mg/mL

Storage Condition: APAF1 antibody can be stored at 4˚C for short term (weeks) . Long term storage should be at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: KIAA0413, Apoptotic protease-activating factor 1APAF-1, CED4, APAF-1,

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Apoptotic protease activating factor 1. Apaf-1 is a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD) , and an ATPase domain (NB-ARC) . Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.