APC11 Antibody | 5737

APC11 Antibody | 5737 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: APC11 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC11.
The immunogen is located within amino acids 70 - 120 of APC11.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB, IF

Application: APC11 antibody can be used for detection of APC11 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 17-201 - 3T3/BALB Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 22 kDa
Observed: 22 kDa

Validation: N/A

Isoform: N/A

Purification: APC11 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: APC11 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: APC11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: APC11 Antibody: APC11, Apc11p, HSPC214, Anaphase-promoting complex subunit 11, Cyclosome subunit 11, APC11

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: APC11 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC11 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C) , a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC11, together with APC2 and Ubc4, form the minimal APC ubiquitin ligase module.