Apo-J/Clusterin | 4325

Apoliprotein J (APO-J), also named Clusterin, is a 75-80 kD disulfide-linked heterodimeric protein. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the a and b chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil a-helices and three predicted amphipathic a-helices. Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.

4325 | Apo-J/Clusterin DataSheet

Biomolecule/Target: ApoJ/Clusterin

Synonyms: CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, , MGC24903, Clusterin, Apolipoprotein J, Apo-J

Alternates names: Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1, Alpha platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor

Taglines: A protein associated withclearance of cellular debris and apoptosis

NCBI Gene ID #: 5154

NCBI Gene Symbol: PDGFA

Gene Source: N/A

Accession #: P04085

Recombinant: No

Source: Plasma

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent stimulation of thymidine uptake by Balb-c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of >1 x 10 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 28.5 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized from PBS, pH 7.5.

Reconstitution Instructions: Reconstitution in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A