Description
APOM Antibody | 45-270 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Dog
Immunogen: The immunogen for this antibody is: PRNQEACELSNN
Research Area: Cancer
Tested Application: E, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Kidney and Liver lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kDa according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes. Recommended concentration, 3-6ug/ml.
Specificiy: N/A
Positive Control 1: Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: APOM, apolipoprotein M, G3a, HSPC336, MGC22400, NG20, NG20-like protein, G3a, NG20
User Note: N/A
BACKGROUND: N/A