ARF6 Antibody | 7699

ARF6 Antibody | 7699 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%) , Chicken: (100%)

Immunogen: ARF6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ARF6.
The immunogen is located within the last 50 amino acids of ARF6.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: ARF6 antibody can be used for detection of ARF6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: ARF6 antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1464 - Rat Liver Tissue Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 19 kDa
Observed: 18 kDa

Validation: N/A

Isoform: N/A

Purification: ARF6 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ARF6 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ARF6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: ARF6 Antibody: ADP-ribosylation factor 6

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The ADP-ribosylation factor 6 (ARF6) is a member of the ARF family, which is part of the RAS superfamily (1) . The ARF proteins are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D (2) . The product of this gene is localized to the plasma membrane (3) , and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling (4) .