Arfaptin 1 (K314) polyclonal Antibody | BS3076

Arfaptin 1 (K314) polyclonal Antibody | BS3076 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC IF

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background: Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Arfaptin 1 (K314) polyclonal Antibody detects endogenous levels of Arfaptin 1 protein.

Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1; Arfaptin1

Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human Arfaptin 1.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: