Argininosuccinate Synthetase (ASS1), E. coli, Recombinant | P1412

Argininosuccinate Synthetase is part of the urea cycle. It catalyzes aspartate, citrulline, and ATP to form argininosuccinate. Mutations in the enzyme cause Citrullinemia, preventing the liver from processing nitrogen to urea. Lack of argininosuccinate synthase has also been observed in pancreatic cancer, liver cancer, melanoma

P1412 | Argininosuccinate Synthetase (ASS1), E. coli, Recombinant DataSheet

Biomolecule/Target :

Synonyms: ASSCTLN1, Citrulline--aspartate ligase, argG,, argininosuccinate synthase 1

Alternates names: ASSCTLN1, Citrulline--aspartate ligase, argG,, argininosuccinate synthase 1

Taglines: Involved in urea cycle that catalyzes aspartate, citrulline, and ATP to form argininosuccinate

NCBI Gene ID #: 947590

NCBI Gene Symbol: argG

Gene Source: E Coli

Accession #: P0A6E4

Recombinant: True

Source: E.coli

Purity by SDS-PAGE: >95%

Assay: SDS-PAGE.

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): This enzyme has a specific activity of ≥ 500 mU/mg based on its ability to convert citrulline to argininosuccinate, which can be detected fluorometrically at Ex/Em = 535/595 using ’s Methionine Assay Kit (K442).

Biological activity:

Results: ≥ 500 mU/mg

Binding Capacity:

Unit Definition: One unit is the amount of enzyme that will hydrolyze 1.0 umole of citrulline to argininosuccinate per minute at pH 7.0 and 37 ÌŠC.

Molecular Weight: 50 kDa with N-terminal 6X-His tag

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute the lyophilized protein in H2O with concentration of 1.0 mU/ml. Incubate the reconstituted protein at 25 °C for 15 minutes.

Amino acid sequence: aa 1 – 447

Handling: Centrifuge the vial prior to opening.

Usage: