ARID1A Antibody | 8173

ARID1A Antibody | 8173 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A.
The immunogen is located within amino acids 580 - 630 of ARID1A.

Research Area: Homeostasis, Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.

Positive Control 1: Cat. No. 1220 - SK-N-SH Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 133, 227, 251 kDa
Observed: 130, 260 kDa

Validation: N/A

Isoform: N/A

Purification: ARID1A antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ARID1A antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ARID1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: AT rich interactive domain 1A (SWI-like) , RP1-50O24.1, B120, BAF250, BAF250a, BM029, C1orf4, ELD, hELD, MRD14, OSA1, hOSA1, P270, SMARCF1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1) . It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2) .