ARID5B Antibody (Center) [APR31547G]

ARID5B Antibody (Center) [APR31547G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ARID5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-418 amino acids from the Central region of human ARID5B.

Cellular Localisation: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00355}.

Molecular Weight: 132375

Clone: Polyclonal

Gene Name: ARID5B

Gene ID: 84159

Function: Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer.

Summary: Tissue Location: Widely expressed, including in liver (at protein level) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: AT-rich interactive domain-containing protein 5B, ARID domain-containing protein 5B, MRF1-like protein, Modulator recognition factor 2, MRF-2, ARID5B, DESRT, MRF2