Aromatase (CYP19A1) Antibody (C-term) [APG01994G]

Aromatase (CYP19A1) Antibody (C-term) [APG01994G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This Aromatase (CYP19A1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-485 amino acids from the C-terminal region of human Aromatase (CYP19A1) .

Cellular Localisation: Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi- pass membrane protein

Molecular Weight: 57883

Clone: Polyclonal

Gene Name: CYP19A1 {ECO:0000303|PubMed:24705274, ECO:0000312|HGNC:HGNC:2594}

Gene ID: 1588

Function: A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3, 17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively (PubMed:27702664, PubMed:2848247) . Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid (PubMed:20385561) . Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1, 10-dehydro 19- nordihydrotestosterone and may play a role in homeostasis of this potent androgen (PubMed:22773874) . Also displays 2-hydroxylase activity toward estrone (PubMed:22773874) . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:20385561, PubMed:22773874) .

Summary: Tissue Location: Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase, CYP19A1, ARO1, CYAR, CYP19