Description
ARPC2 Antibody | 45-011 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: C-EMKTITGKTFSSR
Research Area: Homeostasis
Tested Application: E, WB, IHC, IF
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 34-35kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3kDa according to NP_005722.1) . Recommended concentration: 0.1-0.5ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5ug/ml.Immunofluorescence: :Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10ug/ml.
Specificiy: The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: ARPC2, actin related protein 2/3 complex, subunit 2, 34kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2
User Note: N/A
BACKGROUND: N/A