AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1 | AS10 931

AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1 | AS10 931 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PCaP1 sequence UniProt: Q96262, TAIR: At4g20260

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: antibodies to plasma membrane proteinsPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 2000 (WB)

Molecular weight: 24, 5 | 36 kDa

Confirmed Reactivity: Arabidopsis thaliana, Raphanus sativus (41 kDa), Brassica rapa (42 kDa), Brassica rapa var. glabra Regel (43 kDa), Brassica oleracea var. italica (41 kDa)

Predicted Reactivity: Brassicaceae Species of your interest not listed? Contact us

Not reactive in: Nicotiana benthamiana

Additional Information: It is confirmed that the nock-out mutant (SALK_022955, Col-0 background) has no mRNA or protein of PCaP1. In the immunoblot, crude membrane fractions prepared from wild-type and pcap1 knock-out mutant plants were examined. Therefore the antibody to PCaP1 specifically recognizes PCaP1 and can be used for immunoblotting. For application in immunocytochemistry cross reacting band in proximity of Rubisco has to be removed by incubation of an antibody with a part of memrane with background band. Protocol for preparation of crude membranes This protocol will help to prepare crude membrane fractions from very small amount of plant tissue. (1) Homogenize roots or root tips with the buffer without SDS. (2) Centrifuge the homogenate in Eppendorf tubes at 2 000 rpm for 2 min to remove cell wall, nuclei, plastids and mitochondria. (3) Centrifuge the obtained supernatant at 30 000 g for 20 min to separate soluble components. Please use adequate tubes for ultracentrifugation. (4) Suspend the obtained precipitate (crude membranes) with SDS buffer with DTT or beta-mercaptoethanol. (5) Heat the sample solution obtained the step (4) at 70 C for 5 min. (6) Apply the denatured sample to SDS-PAGE.Courtesy Prof. Msayoshi Maeshima, Nagoya University, Japan

Background: AtPCaP1 is a protein associated with plasma membrane with ability to bind Ca, calmodulin and phosphatidylinositol phoshphates. Alternative names: At4g20260, endomembrane-associated protein.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G20260

Category: Plasma membrane

Research Area: Cell Biology