Additional Information
Size:  | 
                                                    100 µl  | 
                                                
Target:  | 
                                                    Aurora B (pSer227)  | 
                                                
Conjugate:  | 
                                                    ATTO 680  | 
                                                
Research Area:  | 
                                                    Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | Aurora | Cell Signaling | Epigenetics and Nuclear Signaling | Chromatin Modifying Enzymes | Cancer | Cell Cycle  | 
                                                
Alternative Name:  | 
                                                    AIK2 Antibody, ARK2 Antibody, Aurora related Kinase 2 Antibody, IPL1 Antibody, STK1 Antibody, Aurora 1 Antibody  | 
                                                
Category:  | 
                                                    Antibodies  | 
                                                
Product Type:  | 
                                                    Polyclonal Antibody  | 
                                                
Immunogen:  | 
                                                    A phospho-specific peptide corresponding to residues surrounding Ser227 of human Aurora B (AA222-228)  | 
                                                
Immunogen Species:  | 
                                                    Human  | 
                                                
Applications:  | 
                                                    WB, AM  | 
                                                
Host:  | 
                                                    Rabbit  | 
                                                
Isotype:  | 
                                                    N/A  | 
                                                
Species Reactivity:  | 
                                                    Human  | 
                                                
Antibody Dilution:  | 
                                                    WB (1:250); optimal dilutions for assays should be determined by the user.  | 
                                                
Purification:  | 
                                                    Peptide Affinity Purified  | 
                                                
Storage Buffer:  | 
                                                    PBS pH7.4, 50% glycerol, 0.025% Thimerosal  | 
                                                
Concentration:  | 
                                                    N/A  | 
                                                
Specificity:  | 
                                                    Detects 39.28 kDa.  | 
                                                
Storage:  | 
                                                    -20ºC  | 
                                                
Shipping:  | 
                                                    Blue Ice or 4ºC  | 
                                                
Certificate of Analysis:  | 
                                                    A 1:250 dilution of SPC-920 was sufficient for detection of Aurora B (pSer227) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.  | 
                                                
Cellular Localization:  | 
                                                    Nucleus, Cytoplasm, Cytoskeleton  | 
                                                
Tissue Specificity:  | 
                                                    High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase  | 
                                                
Accession Number:  | 
                                                    NP_004208  | 
                                                
Gene ID:  | 
                                                    9212  | 
                                                
Swiss-Prot:  | 
                                                    Q96GD4  | 
                                                
Field of Use:  | 
                                                    For in vitro research use only.  |