AXIN1 Antibody (C-term) [APR03646G]

AXIN1 Antibody (C-term) [APR03646G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This AXIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 710-738 amino acids from the C-terminal region of human AXIN1.

Cellular Localisation: Cytoplasm. Nucleus. Membrane {ECO:0000250|UniProtKB:O35625} Cell membrane {ECO:0000250|UniProtKB:O35625}. Note=MACF1 is required for its translocation to cell membrane (By similarity) . On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684) . {ECO:0000250|UniProtKB:O35625, ECO:0000269|PubMed:17210684}

Molecular Weight: 95635

Clone: Polyclonal

Gene Name: AXIN1

Gene ID: 8312

Function: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:12192039, PubMed:27098453) . Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt- independent JNK signaling pathway (PubMed:12192039) . In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B (PubMed:12192039) . Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (PubMed:16601693) . Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development (PubMed:17210684) . Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (PubMed:17210684) .

Summary: Tissue Location: Ubiquitously expressed.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, WB

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Axin-1, Axis inhibition protein 1, hAxin, AXIN1, AXIN