B-RAF Antibody (S445) [APR32503G]

B-RAF Antibody (S445) [APR32503G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from human B-RAF.

Cellular Localisation: Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

Molecular Weight: 84437

Clone: Polyclonal

Gene Name: BRAF (HGNC:1097)

Gene ID: 673

Function: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable) . Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126) . May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179) .

Summary: Tissue Location: Brain and testis.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, IF, FC

Dilution: IF--1:25 FC--1:25 WB--1:2000 WB--1:1000 IHC-P--1:100~500 IF--1:10~50

Synonyms: Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1