B. subtilis Recombinant, Oxalate oxidase (OxOx) | P1091

Oxalate oxidase is a member of the cupin superfamily. It catalyzes the breakdown of oxalate to hydrogen peroxide and carbon dioxide. Oxalate oxidase and oxalate decarboxylase are two similar enzymes that share similar structural and sequence identity. Both enzymes use oxalate as substrate and manganese ions as cofactors. ’s oxalate oxidase is derived from B. subtilis oxalate decarboxylase by introducing three mutations: S161D, E162A and N163S in the active-site loop.

P1091 | B. subtilis Recombinant Oxalate oxidase OxOx DataSheet

Biomolecule/Target: oxdC

Synonyms: OxO, OxOx, OxO_r

Alternates names: Small inducible cytokine A20 precursor, CCL20, Macrophage inflammatory protein 3 , MIP-3-, Liver and activation- regulated chemokine, CC chemokine LARC, chemokine exodus-1, CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20.

Taglines: Converts oxalate to formate and CO2 in an O(2)-dependent reaction

NCBI Gene ID #: 6364

NCBI Gene Symbol: CCL20

Gene Source: Bacillus subtilis

Accession #: P78556

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by the conversion of 1 µmole of oxalate to CO2 and H2O2 per minute at pH 4.5 and 25C.

Biological activity: Determined by its ability to chemoattract human T cells using a concentration range of 7-70 ng/ml.

Results: 230 mU/mg

Binding Capacity: N/A

Unit Definition: One unit is the amount of enzyme that converts 1 µmole of oxalate to CO2 and H2O2 per minute at pH 4.5 and 25C.

Molecular Weight: 8.0 kDa

Concentration: N/A

Appearance: Freeze dried

Physical form description: Freeze-dried from proprietary buffer

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A