B3GNT6, soluble fragment, Human Recombinant | P1221

­1,3­N­acetylglucosaminyltransferase 6 (3GnT6) transfers N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to N-acetylgalactosamine (GalNAc). 3GnT6 is also known as the core 3 synthase, because it creates the core 3 oligosaccharide structure in the mucin-type O-glycan, GlcNAc1­3GalNAc1­serine/threonine. Core 3 synthase is a type II transmembrane protein, and is localized to stomach, small intestine and colonic tissues. It has long been known that synthesis of the core 3 structure is down-regulated in colonic cancer cells and it has been shown that the expression of 3GnT6 is also down-regulated. Colonic cancer cells transfected with 3GnT6 have reduced metastatic potential compared to non-transfected cells, and when injected into mice show decreased tumor formation. Pancreatic cancer cells transfected with 3GnT6 show a similar phenotype, which has been attributed to the addition of core3 O-glycans to 21 integrin.

P1221 | B3GNT6 soluble fragment Human Recombinant DataSheet

Biomolecule/Target: B3GNT6

Synonyms: Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N acetylglucosaminyltransferase, Core 3 synthase, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6, BGnT-6, Beta-1,3-Gn-T6

Alternates names: Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N acetylglucosaminyltransferase, Core 3 synthase, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6, BGnT-6, Beta-1,3-Gn-T6

Taglines: An important precursor in the biosynthesis of mucin-type glycoproteins

NCBI Gene ID #: 843

NCBI Gene Symbol: CASP10

Gene Source: Human

Accession #: Q92851

Recombinant: Yes

Source: Insect cells

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to 4-Nitrophenyl-N-acetylgalactosamine, as measured with a phosphatase-coupled assay.

Biological activity: Specific activity >100 pmol/min/ug

Results: >100 pmol/min/ug

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 50-55 kDa

Concentration: 100 ug/ml

Appearance: Liquid

Physical form description: Sterile filtered solution in 25 mM Tris pH 7.5 and 150 mM NaCl

Reconstitution Instructions: N/A

Amino acid sequence: N/A