Bacterial DNA Purification Kit II - K1457

K1457 | Bacterial DNA Purification Kit II DataSheet

's Bacterial DNA Purification Kit II provides a rapid and reliable method for isolating high-quality total DNA from a wide variety of bacterial species using spin columns. This kit will isolate all cellular DNA, including plasmid DNA. Up to 1x109 bacterial cells can be processed using a spin column. In this kit, bacterial cells in log-phase are harvested and the bacterial cell wall is digested using lysozyme and Proteinase K. The lysed samples are applied to the spin column. The DNA efficiently binds to the spin column, while proteins and other contaminants are removed. Pure DNA is then eluted in sterile water or elution buffer. Each spin column can bind approximately 100 μg genomic DNA. DNA purified using this kit can be used for downstream applications such as PCR, restriction digestion, and hybridization techniques.

Alternate Name :

Tag Line : Using glass beads and spin columns

Summary : High-quality DNA is purified from bacteria using spin columns

Detection Method : Bacteria is lysed using lysozyme, glass beads and DNA is purified using spin columns.

Sample Type : Bacteria

Species Reactivity :

Applications : PCR, restriction digestion, and hybridization

Features and Benefits : Many downstream applications such as PCR, restriction digestion, and hybridization Rapid, easy and convenient Highly pure, high yield High quality spin columns