Bad (phospho-S112) polyclonal Antibody | BS4222

Bad (phospho-S112) polyclonal Antibody | BS4222 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: IHC

Application Range: IHC: 1:50~1:200

Background: Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-Bad (S112) pAb detects endogenous levels of Bad protein only when phosphorylated at Ser112

Molecular Weight: ~ 24 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: BAD, Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8

Immunogen: Synthetic phosphopeptide derived from human Bad around the phosphorylation site of Serine 112.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Cell Cycle Control G1 S Checkpoint,G Protein-coupled Receptors Signaling to MAPK Erk,Cell Cycle G2 M DNA Damage Signaling Pathway,