Bad Rabbit monoclonal Antibody | BS9832M

Bad Rabbit monoclonal Antibody | BS9832M | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB ICC/IF IHC FC

Application Range: WB: 1:500-1:1000 IHC/ICC/IF: 1:50-1:200 FC: 1:20-1:100

Background: Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This Antibody detects endogenous levels of Bad and does not cross-react with related proteins.

Molecular Weight: ~ 24 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: BAD, Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8

Immunogen: Recombinant Antibody.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: Protein A affinity purified

Pathway: