BAF57 Antibody | 45-315

BAF57 Antibody | 45-315 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse

Immunogen: The immunogen for this antibody is: C-PPTDPIPEDEKKE

Research Area: Cancer

Tested Application: E, WB, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6kDa according to NP_003070.3) . A 48kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 ug/ml. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EB06054 (mock transfection in first lane) , tested by Origene. B) EB06054 staining (0.2ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration, 3-5ug/ml.

Specificiy: N/A

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: SMARCE1, BAF57, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1, mammalian chromatin remodeling complex BRG1-associated factor 57, BAF57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1

User Note: N/A

BACKGROUND: N/A