BAFF Antibody [Buffy-2] | XA-1012

BAFF Antibody [Buffy-2] | XA-1012 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Human, Mouse

Homology: N/A

Immunogen: BAFF (monoclonal Buffy-2) antibody was raised against recombinant human soluble BAFF (aa. 83-285) .

Research Area: Chemokines & Cytokines, Cancer

Tested Application: WB, IHC, Flow

Application: BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line) . Cells were incubated on ice for 30min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μg/mL of MAb to BAFF (Buffy-2) (FITC) . After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Anti BAFF (monoclonal Buffy-2) antibody was purified to ≥95% in SDS-PAGE.

Clonality: Monoclonal

Clone: Buffy-2

Isotype: IgM

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS containing 0.02% sodium azide

Concentration: N/A

Storage Condition: BAFF antibody can be stored at 4˚C, stable for one year.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: N/A