Bax Antibody [6A7] | 99-623

Bax Antibody [6A7] | 99-623 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat, Monkey, Bovine

Homology: N/A

Immunogen: Bax peptide (a.a. 12-24) common to mouse, rat, and human

Research Area: Cancer, Cell Cycle, Obesity

Tested Application: Flow

Application: Bax Antibody [6A7] for use in flow cytometry, immunohistochemistry / immunocytochemistry, electron microscopy, western blotting, immunoprecipitation, and neutralization assays.

Specificiy: Bax

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [6A7]

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

Concentration: 0.1 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: Apoptosis regulator BAX, BCL2 associated X protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Bax is a 20-22 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. In murine thymocytes, it is expressed primarily as a cytoplasmic protein. Over-expression of Bax promotes apoptosis by formation of homodimers and through heterodimerization with Bcl-2, an inhibitor of apoptosis. The monoclonal antibody 6A7 reacts with human, mouse and rat Bax but does not bind the soluble cytosolic form of Bax; however, treatment of cells with non-ionic detergents exposes the epitope and allows binding of 6A7 to monomeric forms of Bax but not Bax complexed with either Bcl-2 or Bcl-xL.