Bcl-2 Antibody (BH3 Domain Specific) [APR06977G]

Bcl-2 Antibody (BH3 Domain Specific) [APR06977G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-110 amino acids from human Bcl.

Cellular Localisation: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein

Molecular Weight: 26266

Clone: Polyclonal

Gene Name: BCL2

Gene ID: 596

Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370) . Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354) . Appears to function in a feedback loop system with caspases (PubMed:11368354) . Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354) . Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974) . May attenuate inflammation by impairing NLRP1- inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785) .

Summary: Tissue Location: Expressed in a variety of tissues.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, IF, WB

Dilution: IHC-P--1:100 WB--1:1000 IF--1:10~50

Synonyms: Apoptosis regulator Bcl-2, BCL2