Bcl-G Antibody | 3165

Bcl-G Antibody | 3165 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (92%)

Immunogen: Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G.
The immunogen is located within the first 50 amino acids of Bcl-G.

Research Area: Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: Although antibody should react with both isoforms, only the Bcl-GS protein has been observed

Positive Control 1: Cat. No. 1215 - U937 Cell Lysate

Positive Control 2: Cat. No. 1416 - Mouse Testis Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Bcl-G Antibody is Ion exchange chromatography purified.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Bcl-G Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Bcl-G antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Bcl-G Antibody: BCLG, BCLG, Apoptosis facilitator Bcl-2-like protein 14, Apoptosis regulator Bcl-G, Bcl2-L-14

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Bcl-G Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.