Bcl-X Antibody [BX006] | 33-071

Bcl-X Antibody [BX006] | 33-071 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat, Pig

Homology: N/A

Immunogen: Amino acids QSNRELVVDFLS (3-14) of the human protien were used as the immunogen for this Bcl-X antibody (2) .

Research Area: Apoptosis

Tested Application: WB, Flow, IHC, IF

Application: Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE) : 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (3)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-X antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. This antibody reacts with both BclXS and BclXL.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: BX006

Isotype: IgG2a

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Bcl-xL Antibody: BCLX, BCL2L, BCLXL, BCLXS, Bcl-X, bcl-xL, bcl-xS, PPP1R52, BCL-XL/S, BCLX, Bcl-2-like protein 1, Apoptosis regulator Bcl-X, Bcl2-L-1

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: This antibody recognizes a protein of 27kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long) , a 241 amino acid protein which suppresses cell death, and XS (short) , a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.